konsult-med

Izolacja retrowirusa limfotropowego T od pacjenta z nabytego niedoboru 

 Retrowirus należący do rodziny niedawno odkrytych ludzkich  wirusów białaczki T- komórkowej (HTLV), ale wyraźnie różniący się od każdego poprzedniego izolatu, został wyizolowany od pacjenta rasy kaukaskiej z oznakami i objawami, które często poprzedzają zespół nabytego niedoboru odporności (AIDS). Wirus ten jest typowym wirusem nowotworu RNA typu C, pączkującym z   błony komórkowej , preferuje magnez dla aktywności odwrotnej transkryptazy i ma wewnętrzny antygen (p25) podobny do p24 HTLV.

Przeciwciała z surowicy tego pacjenta reagują z białkami wirusów z podgrupy HTLV-I, ale antysurowice swoiste dla HTLV-I nie wytrącają białek nowego izolatu. Wirus od tego pacjenta został przeniesiony do limfocytów krwi pępowinowej  , a wirus wytwarzany przez te  komórki  jest podobny do oryginalnego izolatu. Z tych badań wyciągnięto wniosek, że ten wirus, jak również poprzednie izolaty HTLV, należą do ogólnej rodziny retrowirusów T-limfotropowych, które są przenoszone horyzontalnie u ludzi i mogą być zaangażowane w kilka zespołów patologicznych, w tym AIDS.

 

Wykrywanie i izolacja retrowirusa typu C ze świeżych i hodowanych limfocytów pacjenta ze skórnym chłoniakiem T-celwym.

 

Retrovirus particles with sort C morphology had been present in two T-cell lymphoblastoid cell traces, HUT 102 and CTCL-3, and in recent peripheral blood lymphocytes obtained from a affected person with a cutaneous T-cell lymphoma (mycosis fungoides). The cell traces constantly produce these viruses, that are collectively known as HTLV, pressure CR(HTLV(CR)). Initially, the manufacturing of virus from HUT 102 cells required induction with 5-iodo-2′-deoxyuridine, however the cell line grew to become a constitutive producer of virus at its 56th passage. Cell line CTCL-Three has been a constitutive producer of virus from its second passage in tradition.

Each mature and immature furthercellular virus particles had been seen in thin-section electron micrographs of fastened, pelleted cellular materials; every so often, typical sort C budding virus particles had been seen. No type of intracellular virus particle has been seen. Mature particles had been 100-110 nm in diameter, consisted of an electron-dense core surrounded by an outer membrane separated by an electron-lucent area, banded at a density of 1.16 g/ml on a steady 25-65% sucrose gradient, and contained 70S RNA and a DNA polymerase exercise typical of viral reverse transcriptase (RT; RNA-dependent DNA nucleotidyltransferase). Beneath sure circumstances of assay, HTLV(CR) RT confirmed cation choice for Mg(2+) over Mn(2+), distinct from the traits of cellular DNA polymerases purified from human lymphocytes and the RT from most sort C viruses.

Antibodies to cellular DNA polymerase gamma and anti-bodies in opposition to RT purified from a number of animal retroviruses didn’t detectably work together with HTLV(CR) RT underneath circumstances that had been constructive for the respective homologous DNA polymerase, demonstrating a scarcity of shut relationship of HTLV(CR) RT to cellular DNA polymerases gamma or RT of those viruses. Six main proteins, with sizes of roughly 10,000, 13,000, 19,000, 24,000, 42,000, and 52,000 daltons, had been obvious when doubly banded, disrupted HTLV(CR) particles had been chromatographed on a NaDodSO(4)/polyacrylamide gel. The variety of these particle-associated proteins is in step with the anticipated proteins of a retrovirus, however the sizes of some are distinct from these of most recognized retroviruses of the primate subgroups.

 

U myszy z niedoborem interleukiny 10 rozwija się przewlekłe zapalenie jelit.

 

Interleukin-10 (IL-10) impacts the expansion and differentiation of many hemopoietic cells in vitro; specifically, it’s a potent suppressor of macrophage and T cell capabilities. In IL-10-deficient mice, generated by gene focusing on, lymphocyte improvement and antibody responses are regular, however most animals are development retarded and anemic and endure from power enterocolitis. Alterations in gut embody in depth mucosal hyperplasia, inflammatory reactions, and aberrant expression of main histocompatibility advanced class II molecules on epithelia.

In distinction, mutants saved underneath particular pathogen-free circumstances develop solely a neighborhood irritation restricted to the proximal colon. These outcomes point out that the bowel irritation within the mutants originates from uncontrolled immune responses stimulated by enteric antigens and that IL-10 is a vital immunoregulator within the intestinal tract.

konsult-med
konsult-med

Prosta technika ilościowego określania niskich poziomów uszkodzeń DNA w poszczególnych komórkach.

Human lymphocytes had been both uncovered to X-irradiation (25 to 200 rads) or handled with H2O2 (9.1 to 291 microM) at Four levels C and the extent of DNA migration was measured utilizing a single-cell microgel electrophoresis method underneath alkaline circumstances. Each brokers induced a big enhance in DNA migration, starting on the lowest dose evaluated.

Migration patterns had been comparatively homogeneous amongst cells uncovered to X-rays however heterogeneous amongst cells handled with H2O2. An evaluation of restore kinetics following publicity to 200 rads X-rays was performed with lymphocytes obtained from three people. The majority of the DNA restore occurred throughout the first 15 min, whereas the entire restore was primarily full by 120 min after publicity. Nonetheless, some cells demonstrated no restore throughout this incubation interval whereas different cells demonstrated DNA migration patterns indicative of extra harm than that induced by the preliminary irradiation with X-rays. This system seems to be delicate and helpful for detecting harm and restore in single cells.

Zaangażowanie immunoinhibitorowego receptora PD-1 przez nowego członka rodziny B7 prowadzi do negatywnej regulacji aktywacji limfocytów.

 

PD-1 is an immunoinhibitory receptor expressed by activated T cells, B cells, and myeloid cells. Mice poor in PD-1 exhibit a breakdown of peripheral tolerance and exhibit a number of autoimmune options. We report right here that the ligand of PD-1 (PD-L1) is a member of the B7 gene household. Engagement of PD-1 by PD-L1 results in the inhibition of T cell receptor-mediated lymphocyte proliferation and cytokine secretion. As well as, PD-1 signaling can inhibit a minimum of suboptimal ranges of CD28-mediated costimulation. PD-L1 is expressed by antigen-presenting cells, together with human peripheral blood monocytes stimulated with interferon gamma, and activated human and murine dendritic cells.

As well as, PD-L1 is expressed in nonlymphoid tissues resembling coronary heart and lung. The relative ranges of inhibitory PD-L1 and costimulatory B7-1/B7-2 indicators on antigen-presenting cells might decide the extent of T cell activation and consequently the edge between tolerance and autoimmunity. PD-L1 expression on nonlymphoid tissues and its potential interplay with PD-1 might subsequently decide the extent of immune responses at websites of irritation.

 

TARGATT? Knock-in iPSC Generation (Master Cell Line)

AST-1100 1 vial of 1X10^6 cells Ask for price
Description: 6 month

TARGATT? Knock-in Mouse Cell Line Generation Kit (Master Cell Line)

AST-7001 1 Kit Ask for price
Description: 6 month

HEK-293T cells

T0011002 One Frozen vial
EUR 455

TARGATT? Knock-in CHO Generation Kit (Master Cell Line)

AST-1200 1 Kit Ask for price
Description: 12 month

TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line)

AST-1300 1 Kit Ask for price
Description: 12 month

HEK-293T Telomerase Over-Expressing Cell Pellet

abx069991-1Pellet 1 Pellet
EUR 398

LCK 3'UTR GFP Stable Cell Line

TU062323 1.0 ml
EUR 1394

LCK 3'UTR Luciferase Stable Cell Line

TU012323 1.0 ml
EUR 1394

Lck 3'UTR GFP Stable Cell Line

TU256973 1.0 ml Ask for price

Lck 3'UTR Luciferase Stable Cell Line

TU206973 1.0 ml Ask for price

Lck 3'UTR GFP Stable Cell Line

TU160948 1.0 ml Ask for price

Lck 3'UTR Luciferase Stable Cell Line

TU110948 1.0 ml Ask for price

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out

HR100PA-1 10 ug
EUR 938

293/GFP Cell Line

AKR-200 1 vial
EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572

Human Tyrosine-protein kinase Lck (LCK)

1-CSB-EP012806HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Tyrosine-protein kinase Lck(LCK) expressed in E.coli

Human Tyrosine-protein kinase Lck (LCK)

1-CSB-EP012806HUa2
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Tyrosine-protein kinase Lck(LCK) expressed in E.coli

293T Transfection Kit (1 mL)

P902 - Ask for price

293T Transfection Kit (0.2 mL)

P902S - Ask for price

293T Transfection Kit (1 mL)

P903 - Ask for price

293T Transfection Kit (0.2 mL)

P903S - Ask for price

Microplate Swing-Out Centrifuge

abx725026-1Unit 1 Unit
EUR 1476

Clinical Swing-Out Centrifuge

abx725027-1Unit 1 Unit
EUR 1476

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 572
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 572
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 572
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Gene Knock-Out HR Targeting Vector w/Single Selection Marker (Blasticidin) and Negative Selection (TK) Against Random Integration

HR720PA-1 10 µg
EUR 1145

Lck/ Rat Lck ELISA Kit

ELI-38801r 96 Tests
EUR 886

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (GFP+Puro) and Negative Selection (TK) Against Random Integration

HR700PA-1 10 µg
EUR 1145

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration

HR710PA-1 10 µg
EUR 1145

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Human Tyrosine- protein kinase Lck, LCK ELISA KIT

ELI-31641h 96 Tests
EUR 824

Human Tyrosine protein kinase Lck(LCK) ELISA kit

E01T0623-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Tyrosine protein kinase Lck(LCK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Tyrosine protein kinase Lck(LCK) ELISA kit

E01T0623-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Tyrosine protein kinase Lck(LCK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Tyrosine protein kinase Lck(LCK) ELISA kit

E01T0623-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Tyrosine protein kinase Lck(LCK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

LCK sgRNA CRISPR Lentivector set (Human)

K1201901 3 x 1.0 ug
EUR 339

Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1?-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2]

HR210PA-1 10 ug
EUR 1145

Monoclonal p56lck / LCK Antibody (clone Lck-01), Clone: Lck-01

APR17729G 0.05mg
EUR 484
Description: A Monoclonal antibody against Human p56lck / LCK (clone Lck-01). The antibodies are raised in Mouse and are from clone Lck-01. This antibody is applicable in WB and IHC-P, ICC, IP, Flo

Tyrosine-Protein Kinase Lck (LCK) Antibody

abx159593-100ul 100 ul
EUR 467

LCK Antibody

1-CSB-PA009795
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LCK. Recognizes LCK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000

LCK Antibody

1-CSB-PA009798
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LCK. Recognizes LCK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/40000

LCK Antibody

1-CSB-PA009802
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LCK. Recognizes LCK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000

LCK Antibody

1-CSB-PA012806GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against LCK. Recognizes LCK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

LCK Antibody

1-CSB-PA012806LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LCK. Recognizes LCK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF, IP; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

LCK Antibody

1-CSB-PA003142
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LCK. Recognizes LCK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000

Lck Inhibitor

HY-12072 100mg
EUR 2019

Lck Antibody

35363-100ul 100ul
EUR 390

Lck Antibody

35446-100ul 100ul
EUR 390

LCK Antibody

32644-100ul 100ul
EUR 252

LCK siRNA

20-abx922340
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

LCK siRNA

20-abx922341
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

LCK siRNA

20-abx902944
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

Lck Inhibitor

A3539-1 1 mg
EUR 154
Description: Lck Inhibitor is a small-molecule inhibitor of with IC50 value of 7 nM [1].The lymphocyte specific kinase which expressed in NK cells and T-cells is a member of the Src kinase family.

Lck Inhibitor

A3539-5 5 mg
EUR 222
Description: Lck Inhibitor is a small-molecule inhibitor of with IC50 value of 7 nM [1].The lymphocyte specific kinase which expressed in NK cells and T-cells is a member of the Src kinase family.

LCK Antibody

AF5412 200ul
EUR 304
Description: LCK Antibody detects endogenous levels of total LCK.

Lck Antibody

AF6100 200ul
EUR 304
Description: Lck Antibody detects endogenous levels of total Lck.

Lck Antibody

AF6101 200ul
EUR 304
Description: Lck Antibody detects endogenous levels of total Lck.

Lck Antibody

AF7694 200ul
EUR 376
Description: Lck Antibody detects endogenous levels of Lck.

Lck Antibody

AF7695 200ul
EUR 376
Description: Lck Antibody detects endogenous levels of Lck.

LCK Antibody

AF7696 200ul
EUR 376
Description: LCK Antibody detects endogenous levels of LCK.

LCK Antibody

ABD6900 100 ug
EUR 438

LCK Antibody

ABF5412 100 ug
EUR 438

LCK Antibody

DF6900 200ul
EUR 304
Description: LCK Antibody detects endogenous levels of total LCK.

Lck Antibody

BF0456 200ul
EUR 376
Description: Lck antibody detects endogenous levels of total Lck.

anti-LCK

LF-PA0206 100 ul
EUR 334
Description: Rabbit polyclonal to LCK

Lck Antibody

ABF6100 100 ug
EUR 438

Lck Antibody

ABF6101 100 ug
EUR 438

anti-Lck

YF-PA12927 100 ug
EUR 403
Description: Rabbit polyclonal to Lck

Lck, Active

7733-5
EUR 370

Lck Antibody

3498-100
EUR 316

Lck Antibody

3498-30T
EUR 146

LCK antibody

20R-1997 50 ug
EUR 281
Description: Rabbit polyclonal LCK antibody

LCK antibody

20R-2166 50 ug
EUR 281
Description: Rabbit polyclonal LCK antibody

LCK antibody

20R-2987 100 ul
EUR 393
Description: Rabbit polyclonal LCK antibody

Lck antibody

20R-1612 100 ug
EUR 673
Description: Rabbit polyclonal Lck antibody

Lck antibody

20R-1613 100 ug
EUR 705
Description: Rabbit polyclonal Lck antibody