Zestaw do kolorymetrycznego testu proliferacji komórek zestaw do liczenia komórek-8
Tło
Proliferacja komórek zostanie prawdopodobnie wykryta wieloma metodami. Proliferacja komórek powoduje wzrost w ciągu całego ciągu dehydrogenaz mitochondrialnych, które rozszczepiają sól tetrazoliową do formazanu.
Pakiet testu kolorymetrycznego WST Cell Proliferation Assay Package plus zapewnia jedną z najlepszych i najdelikatniejszych technik ilościowego oznaczania proliferacji i żywotności komórek. Proliferacja komórek powoduje wzrost ilości normalnego barwnika formazanu, który jest wprost proporcjonalny do liczby rezydujących komórek i prawdopodobnie zostanie określony ilościowo przez pomiar absorbancji decyzji barwnika przy 440 nm przy użyciu czytnika płytek do mikromiareczkowania.
Sprzęt może być wykorzystany do analizy proliferacji komórek w odpowiedzi na związki farmaceutyczne, związki cytotoksyczne, takie jak leki przeciwnowotworowe i wiele różnych brokerów toksycznych. Test można stosować do wykrywania proliferacji komórek w odpowiedzi na cytokiny, mitogeny, części postępu i witaminy odżywcze i tak dalej.
Test może być bardzo łatwy i prosty, wystarczy dodać i odczytać, nie wymaga rozpuszczania, płukania ani zbierania, i jest regularny, wcześniejszy i bardziej delikatny niż testy oparte na MTS, MTT lub XTT. Twój cały test prawdopodobnie zostanie przeprowadzony na płytce do mikromiareczkowania i jest odpowiednio skorelowany z testem włączania [3H]-tymidyny.
Opis
Nasz produkt Cell Counting Package deal-8 (CCK-8) jest dodatkowym pomocnym i delikatnym podejściem do oceny ilości komórek i testu proliferacji totoksyczności niż wcześniejsze strategie. Przekładnia wykorzystuje wyjątkowo rozpuszczalną w wodzie sól tetrazoliową WST-8, która wytwarza rozpuszczalny w wodzie barwnik formazanowy przy niskich kosztach w obecności mediatora elektronowego. Ilość formazanu generowanego przez dehydrogenazy jest wprost proporcjonalna do liczby znajdujących się komórek. Czułość detekcji CCK-Eight jest większa niż zupełnie innych soli tetrazoliowych przypominających MTT, XTT, MTS czy WST-1.
Wybory i właściwości
| Choices | Fast and helpful Straightforward one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The technique is really easy that requires no washing/harvesting/or solubilization steps and is faster and additional delicate than MTT/XTT/or MTS-based assays. Your whole assay will probably be carried out straight in a 96-well plate. |
| Supply | Gel pack |
| Storage Circumstances | Retailer at -20°C. |
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 CCK-8 Cell Counting Kit |
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| A311-02 | Vazyme | 1000 rxn | EUR 234 |
) Cell Counting Kit-8 (CCK-8) |
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| B34302 | Bimake | 5 mL (500 ractions) | EUR 130 |
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Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading. |
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Cell Counting Kit-8 (CCK-8) |
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| B34304 | Bimake | 25 mL (2500 ractions) | EUR 315 |
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Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading. |
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Cell Counting Kit-8 (CCK-8) |
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| AR1160 | BosterBio | 5 mL (for 500 assays, 10μL per well) | EUR 131 |
 Cell Viability Assay Kit) Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit |
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| abx090677-500tests | Abbexa | 500 tests | EUR 394 |
 Bacterial Counting Colorimetric Assay Kit |
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| K511-2500 | Biovision | EUR 620 | |
Bacterial Counting Colorimetric Assay Kit |
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| K511-500 | Biovision | EUR 349 | |
 CORNING COUNTING CHAMBER FOR CORNING CELL COUNTER |
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| 480200 | CORNING | 1/pk | EUR 142 |
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Description: Lab Equipment; Cell Counters |
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 Frit Kit |
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| FRIT-KIT | Next Advance | 1each | EUR 124 |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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 Column Packing Kit |
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| PACK-KIT | Next Advance | 1pack | EUR 1035 |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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 PCR Mycoplasma Detection Kit |
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| M034-Kit | TOKU-E | Kit | EUR 266 |
 CytoSelect BrdU Cell Proliferation ELISA Kit |
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| CBA-251 | Cell Biolabs | 96 assays | EUR 531 |
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Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation. |
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) PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN400A-KIT | SBI | 1 Kit | EUR 2798 |
) PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN300A-KIT | SBI | 1 Kit | EUR 2798 |
 HIF-1 Alpha Cell Based ELISA Kit |
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| CBA-281 | Cell Biolabs | 96 assays | EUR 612 |
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Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat. |
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 CytoSelect 48-Well Cell Contraction Assay Kit |
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| CBA-5021 | Cell Biolabs | 48 assays | EUR 635 |
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Description: Cell Biolabs? Cell Contraction Assays (Floating Matrix Model) provide a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction. |
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 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS400A-KIT | SBI | 1 kit (10 rxn) | EUR 1110 |
) PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN410A-KIT | SBI | 1 Kit | EUR 4335 |
) PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN412A-KIT | SBI | 1 Kit | EUR 4335 |
 CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV100PA-KIT | SBI | 1 Kit | EUR 1132 |
 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV105PA-KIT | SBI | 1 Kit | EUR 1132 |
 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV120PA-KIT | SBI | 1 Kit | EUR 1132 |
 MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV125PA-KIT | SBI | 1 Kit | EUR 1132 |
 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS700A-KIT | SBI | 10 rxn | EUR 1132 |
 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS720A-KIT | SBI | 10 rxn | EUR 1132 |
 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS740A-KIT | SBI | 10 rxn | EUR 1132 |
) CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps) |
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| CBA-155 | Cell Biolabs | 5 preps | EUR 798 |
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Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis. |
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 ELISA Kit) CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit |
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| CBA-254 | Cell Biolabs | 96 assays | EUR 560 |
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Description: Cell Biolabs? CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts. The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mLPCNA. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. |
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) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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| CAS510A-KIT | SBI | 1 Kit | EUR 805 |
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV200PA-KIT | SBI | 1 Kit | EUR 1132 |
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV205PA-KIT | SBI | 1 Kit | EUR 1132 |
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV220PA-KIT | SBI | 1 Kit | EUR 1132 |
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV225PA-KIT | SBI | 1 Kit | EUR 1132 |
 Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS750A-KIT | SBI | 10 rxn | EUR 1132 |
 Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS770A-KIT | SBI | 10 rxn | EUR 1132 |
 Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS790A-KIT | SBI | 10 rxn | EUR 1132 |
 and Fast ligase (2.5ul)]) Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
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| CAS9LIG-KIT | SBI | 1 Kit | EUR 153 |
) PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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| PIN320A-KIT | SBI | 1 Kit | EUR 4941 |
, Colorimetric) CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
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| CBA-135 | Cell Biolabs | 96 assays | EUR 821 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
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| CBA-135-5 | Cell Biolabs | 5 x 96 assays | EUR 3356 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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, Fluorometric) CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric |
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| CBA-140 | Cell Biolabs | 96 assays | EUR 856 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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